Researchers use bench-stable nucleoside 3′-phosphorofluoridates to enable rapid coupling and automated synthesis of oligonucleotides
Oligonucleotide synthesis is fundamental to genetic analysis, biological research, and nucleic acid therapeutics. However, current methods rely on moisture-sensitive building blocks and require a dedicated oxidation step with each chain elongation, making it operationally complex. To address this, researchers from Japan have developed a new approach using phosphorofluoridate building blocks, enabling efficient oxidation-free oligonucleotide synthesis. This advance simplifies the synthetic workflow, offering a faster and more reliable platform for nucleotide chemistry.
Efficient, oxidation-free, P(V)-based oligonucleotide synthesis strategy
