Division of Molecular Genetics

Main Research AreasResearch ProjectsMemberPublications

 

Main Research Areas

  • molecular mechanisms of insulin action
  • molecular mechanisms of the shedding of insulin receptor ectodomain and diabetes mellitus

 

Research Projects

1."Stimulation of Glucose uptake by insulin in insulin target tissues"

〔Insulin-stimulated Glucose Transporter Type 4 (GLUT4) translocation.〕

Stimulation of Glucose uptake by insulin in insulin target tissue

2."c-myc tagged GLUT4"(J.Biol.Chem. 1993)

c-myc tagged GLUT4

3."Detection of GLUT4myc on the cell surface"(J.Biol.Chem. 1993)

Detection of GLUT4myc on the cell surface

4."Stimulation of glucose uptake via Gq"(J.Biol.Chem. 1996, Diabetes 1998)

Stimulation of glucose uptake via Gq

5."Molecular mechanisms of the shedding of insulin receptor ectodomain and diabetes mellitus "(Diabetes 2007)

Regarding the mechanisms of soluble IR α generation, it has been proposed that the hypothesis that insulin receptors on the plasma membrane receive the “shedding”, which mean that a proteinase activating by a stimuli (e.g. hyperglycemia) cleave insulin receptor, like other membrane receptors such as TNF receptor, EGF receptor and IL-6 receptor.

Molecular mechanisms of the shedding of insulin receptor ectodomain and diabetes mellitus

 

Member

Professor Yousuke EbinaProfessor
Yousuke Ebina
TEL : 088-633-7435
E-mail : ebina@ier.tokushima-u.ac.jp
Associate Professor Tomoyuki YuasaAssociate Professor
Tomoyuki Yuasa
TEL : 088-633-7436
E-mail : yuasa@ier.tokushima-u.ac.jp
Assistant Professor Shuhei IshikuraAssistant Professor
Shuhei Ishikura
TEL : 088-633-7436
E-mail : ishikura@ier.tokushima-u.ac.jp
Assistant Professor Kikuko AmoAssistant Professor
Kikuko Amo
TEL : 088-633-7436
E-mail : amo@ier.tokushima-u.ac.jp

 

Publications

  1. IRS1-Independent Defects Define Major Nodes of Insulin Resistance Cell Metabolism, 7, 421-433, (07 May 2008)
    Kyle L. Hoehn, Cordula Hohnen-Behrens, Anna Cederberg, Lindsay E. Wu, Nigel Turner, Tomoyuki Yuasa, Yousuke Ebina, and David E. James
  2. Soluble insulin receptor ectodomain is elevated in the plasma of patients with diabetes mellitus.
    Diabetes., 56(8):2028-2035. (2007)
    Toshiyuki Obata, Ichiro Yokota, Kazuhiro Yokoyama, Eiji Okamoto, Yoshiko Kanezaki, Yoshinori Tanaka, Hiroshi Maegawa, Kiyoshi Teshigawara, Fumiko Hirota, Tomoyuki Yuasa, Kazuhiro Kishi, Atsushi Hattori, Seiichi Hashida, Kazuhiko Masuda, Mitsuru Matsumoto, Toshio Matsumoto, Atsunori Kashiwagi, and Yousuke Ebina
  3. Intra-islet insulin suppresses glucagon release via GABA-GABA(A) receptor system.
    Cell Metabolism. 3(1):47-58. (2006)
    Elaine Xu, Mohan Kumar, Yi Zhang, William Ju, Toshiyuki Obata T, Nina Zhang, Shiying Liu, Anna Wendt, Shaoping Deng, Yousuke Ebina, Michael B.Wheeler, Matthias Braun, Qinghua Wang
  4. Platelet-derived growth factor stimulates glucose transport in skeletal muscles of transgenic mice specifically expressing PDGF receptor in the muscle, but does not affect blood glucose levels
    Diabetes, 53, 2776-2786 (2004)
    Tomoyuki Yuasa, Rei Kakuhata, Kazuhiro Kishi, Toshiyuki Obata, Yasuo Shinohara, Yoshimi Bando, Keisuke Izumi, Fumiko Kajiura, Mitsuru Matsumoto, Yousuke Ebina
  5. Increased insulin sensitivity and hypoinsulinemia in APS knockout mice Diabetes 52, 2657-2665 (2003)
    Asako Minami, Masanori Iseki, Kazuhiro Kishi, Miao Wang, Makoto Ogura, Noboru Furukawa, Sanae Hayashi, Mizuki Yamada, Toshiyuki Obata, Yukari Takeshita, Yutaka Nakaya, Yoshimi Bando, Keisuke Izumi, Shonna A. Moodie, Fumiko Kajiura, Mitsuru Matsumoto, Kiyoshi Takatsu, Satoshi Takaki and Yousuke Ebina
  6. Use of RNA-interference-mediated gene silencing and adenoviral overexpression to Elucidate the roles of AKT/PKB-isoforms in insulin actions
    J. Biol. Chem. 278:28312-28323(2003)
    Toshiyuki Obata, Takashi Katome, Rie Matsushima, Norihisa Masuyama, Lewis C. Cantley, Yukiko Gotoh, Kazuhiro Kishi, Hiroshi Shiota, and Yousuke Ebina
  7. Impaired glucose tolerance in mice with a targeted impairment of insulin action in muscle and adipose tissue
    Nature Genetics. 20, 294-298 (1998)
    Davide Lauro, Yoshiaki Kido, Arthur L. Castle, Mary-Jane Zarnowski, Hideki Hayashi, Yousuke Ebina and Domenico Accili
  8. Bradykinin directly triggers GLUT4 translocation via an insulin-independent pathway
    Diabetes 47, 550-558 (1998)
    Kazuhiro Kishi, Naoko Muromoto, Yutaka Nakaya, Ikuko Miyata, Akifumi Hagi, Hideki Hayashi, and Yousuke Ebina
  9. Gq-coupled receptors transmit the signal for GLUT4 translocation via an insulin-independent pathway
    J. Biol. Chem. 271, 26561-26568 (1996) Kazuhiro Kishi, Hideki Hayashi, Lihong Wang, Seika Kamohara, Keisuke Tamaoka, Takao Shimizu, Fumitaka Ushikubi, Shuh Narumiya, and Yousuke Ebina
  10. Platelet-derived growth factor triggers translocation of the insulin-regulatable glucose transporter (type 4) predominantly through phosphatidylinositol 3-kinase binding sites on the receptor
    Proc. Natl. Acad. Sci. USA 92, 1077-1081 (1995)
    Seika Kamohara, Hideki Hayashi, Mikio Todaka, Fumihiko Kanai, Kazuo Ishii, Takanobu Imanaka, Jaime A. Escobedo, Lewis T. Williams and Yousuke Ebina
  11. Direct demonstration of insulin-induced GLUT4 translocation to the surface of intact cells by insertion of a c-myc epitope into an exofacial GLUT4 domain
    J. Biol. Chem. 268, 14523-14526 (1993)
    Fumihiko Kanai, Yasuhiko Nishioka, Hideki Hayashi, Seika Kamohara, Mikio Todaka, and Yousuke Ebina
  12. Insulin-resistant diabetes associated with partial deletion of insulin-receptor gene Lancet 335, 1179-1181 (1990)
    Fumio Shimada, Masato Taira, Yoshifumi Suzuki, Naotake Hashimoto, Osamu Nozaki, Masanori Taira, Masamiti Tatibana, Yousuke Ebina, Masato Tawata, Toshimasa Onaya, Hideichi Makino, and Sho Yoshida
  13. Human diabetes associated with a deletion of the tyrosine kinase domain of the insulin receptor
    Science 245, 63-66 (1989)
    Masato Taira, Masanori Taira, Naotake Hashimoto, Fumio Shimada, Yoshifumi Suzuki, Azuma kanatsuka, Fumiko Nakamura, Yousuke Ebina, Masamiti Tatibana, Hideichi Makino, and Sho Yoshida
  14. Replacement of lysine residue 1030 in the putative ATP-binding region of the insulin receptor abolishes insulin- and antibody-stimulated glucose uptake and receptor kinase activity
    Proc. Natl. Acad. Sci. USA 84, 704-708 (1987)
    Yousuke Ebina, Eiichi Araki, Masato Taira, Fumio Shimada, Masataka Mori, Charles S. Craik, Kenneth Siddle, Sarah B. Pierce, Richard A. Roth, and William J. Rutter
  15. The human insulin receptor cDNA: The structural basis for hormone-activated transmembrane signalling
    Cell 40, 747-758 (1985)
    Yousuke Ebina, Leland Ellis, Kurt Jarnagin, Marc Edery, Laszlo Graf, Eric Clauser, Jing-hsiung Ou, Frank Masiarz, Y. W. Kan, I. D. Goldfine, Richard A. Roth, and William J. Rutter