X-ray crystallography facility

Main Research AreasResearch ProjectsMemberPublications

 

Main Research Areas

  • Mechanisms of transposition of non-LTR retrotransposon
  • Structure of zinc-finger type transcription factor
  • Structure-based drug design

 

A brand-new X-ray analysis system Rigaku FR-E+ (left)

Endonuclease domain of retrotransposon (R1Bm)(right)

A brand-new X-ray analysis system Rigaku FR-E+ (left)
Endonuclease domain of retrotransposon (R1Bm)(right)

 

Research Projects

In the post-genomic era, the elucidation of physiological roles of proteins is of utmost importance. To address the issue, we use the x-ray crystallographic approaches to solve the protein structure, and to elucidate how the proteins work and/or interact with other proteins/subunits. With one of the brightest in-house x-ray generators available (Rigaku FR-E+), we try to promote our own studies on various fields of life sciences as well as collaborative studies with other groups within and outside of the university.

 

Member

Associate Professor Nobuo Maita Associate Professor
Nobuo Maita
Nobuo Maita
TEL : 088-633-9414
E-mail : nmaita@ier.tokushima-u.ac.jp

 

Publications

  1. Fujiwara, K., Maita, N., Hosaka, H., Okamura-Ikeda, K., Nakagawa, A. & Taniguchi, H. Global conformational change associated with the two-step reaction catalyzed by Escherichia coli lipoate-protein ligase A. J. Biol. Chem., in press, 2010.
  2. Maita, N., Nyirenda, J., Igura, M., Kamishikiryo, J. & Kohda, D. Comparative structural biology of Eubacterial and Archaeal oligosaccharyltransferases. J. Biol. Chem., 285: 4941-4950, 2010.
  3. Igura, M., Maita, N., Kamishikiryo, J., Yamada, M., Obita, T., Maenaka, K & Kohda, D. Structure-guided identification of a new catalytic motif of oligosaccharyltransferase. EMBO J., 27: 234-243, 2008.
  4. Maita, N., Aoyagi, H., Osanai, M., Shirakawa, M. & Fujiwara, H. Characterization of the sequence specificity of the R1Bm endonuclease domain by structural and biochemical studies. Nucl. Acids Res., 35: 3918-3927, 2007.
  5. Baba, D., Maita, N., Jee, J.G., Uchimura, Y., Saitoh, H., Sugasawa, K., Hanaoka, F., Tochio, H., Hiroaki, H. & Shirakawa, M. Crystal structure of thymine DNA glycosylase conjugated to SUMO-1. Nature, 435: 979-982, 2005.
  6. Maita, N., Anzai, T., Aoyagi, H., Mizuno, H. & Fujiwara, H. Crystal structure of the endonuclease domain encoded by the telomere-specific long interspersed nuclear element, TRAS1. J. Biol. Chem., 279: 41067-41076, 2004.