Bio Ri Facility

Main Research AreasResearch ProjectsMemberPublications

 

Main Research Areas

  • It is possible to conduct gene engineering researches using radio isotopes(P3 level lab). A staff at associate professor level is supervising the activity.
  • Organelle biogenesis and membrane traffic in disease

 

Research Projects

1.Molecular mechanism on insulin-stimulated Glut4 translocation

Insulin rapidly increases glucose uptake into adipose and muscle tissues. This increase is caused by a rise of insulin-regulated glucose transporter (Glut4) in the plasma membrane, which is achieved by insulin-stimulated movement of intracellular vesicles containing Glut4. In unstimulated adipocytes, Glut4 is excluded from the plasma membrane by slow exocytosis and rapid endocytosis and cycles between the plasma membrane and multiple intracellular compartments. Once stimulated by insulin, the exocytosis of Glut4-containing vesicles is accelerated and the level of Glut4 in the plasma membrane increases. We have identified a novel factor required for the generation of insulin-responsive Glut4 vesicles.

Insulin-stimulated Glut4 translocation

 

Biogenesis of endoplasmic reticulum and Golgi apparatus mediated by AAA-ATPase p97

The endoplasmic reticulum and the Golgi apparatus are important for synthesis, posttranslational processing, and sorting of enzymes. We have investigated a molecular mechanism of membrane-fusion mediated by AAA-ATPase p97, which is required for the biogenesis of ER and Golgi, and identified novel essential factors, VCIP135 and p37.

Insulin-stimulated Glut4 translocation

 

Member

Associate Professor Keiji UCHIYAMAAssociate professor
Keiji Uchiyama
Keiji Uchiyama
TEL : 088-633-7436
E-mail : ku200@ier.tokushima-u.ac.jp

 

Publications

  1. Yuasa, T., Uchiyama, K., Ogura, Y., Kimura, M., Teshigawara T., Hosaka., T., Tanaka, Y., Obata, T., Sano, H., Kishi, K. and Ebina, Y. The Rab GTPase-activating protein AS160 as a common regulator of insulin- and Gαq-mediated intracellular GLUT4 vesicle distribution. Endocrine J. 56: 345-359, 2009
  2. Uchiyama, K., Totsukawa, G., Puhka, M., Kaneko, Y., Jokitalo, E., Dreveny, I., Beuron, F., Zhang, X., Freemont, P., and Kondo, H. p37 is a p97 adaptor required for Golgi and ER biogenesis in interphase and at the end of mitosis. Developmental Cell. 11: 803-816, 2006
  3. Kano, F., Kondo, H., Yamamoto, A., Kaneko, Y., Uchiyama, K., Hosokawa, N., Nagata, K., and Murata, M. NSF/SNAPs and p97/p47/VCIP135 are sequentially required for cell cycle-dependent reformation of the ER network. Genes Cells. 10: 989-999, 2005
  4. Uchiyama, K. and Kondo, H. p97/p47-Mediated Biogenesis of Golgi and ER. J. Biochem. 137: 115-119, 2005
  5. Yuan, X., Simpson, P., Mckeown, C., Kondo, H., Uchiyama, K., Wallis, R., Dreveny, I., Keetch, C., Zhang, X., Robinson, C., Freemont, P. and Matthews, S. Structure, dynamics and interactions of p47, a major adaptor of the AAA ATPase, p97. EMBO J. 23: 1463-1473, 2004
  6. Dreveny I., Kondo H., Uchiyama K., Shaw A., Zhang X., and Freemont P. Structural basis of the interaction between the AAA ATPase p97/VCP and its adaptor protein p47. EMBO J. 23: 1030-1039, 2004
  7. Uchiyama K., Jokitalo E., Lindman M., Jackman M., Kano F., Murata M., Zhang X. and Kondo H. The localization and phosphorylation of p47 are important for Golgi disassembly-assembly during the cell cycle. J. Cell Biol. 161: 1067-1079, 2003
  8. Uchiyama K., Jokitalo E., Kano F., Murata M., Zhang X., Canas B., Newman R., Rabouille C., Pappin D., Freemont P., and Kondo H. VCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for Golgi and ER assembly in vivo. J. Cell Biol. 159: 855-866, 2002