【研究成果報告】低コストかつ環境低負荷型バイオリファイナリーシステムの開発

2017年12月13日

平成29年度 若手研究者学長表彰 研究成果報告

 

報告者

大学院社会産業理工学研究部生物資源産業学域・講師・淺田元子

 

研究タイトル

低コストかつ環境低負荷型バイオリファイナリーシステムの開発

 

研究概要

近年、化石資源枯渇や地球温暖化の問題を解決するための技術開発や未利用資源の有効利用による低炭素社会の構築が求められる中、セルロース系バイオマスからバイオフューエルだけでなく、バイオマテリアルを統合的に生産するバイオリファイナリーシステムの開発的研究に関心が集っている。本研究は、既往技術の問題点であったバイオリファイナリーシステムにおける①汚染物発生の削減、②糖化段階における酵素(セルラーゼ)の低コスト生産と③全構成成分の有効利用法の開発という課題を解決するために、高活性水蒸気処理および粉砕処理、水と有機溶媒を用いた抽出分離、古細菌由来耐熱性酵素を用いた加水分解および種々の変換法から成るバイオリファイナリーシステムの開発を行った。本システムにより、低コストかつ環境低負荷でバイオエタノールやバイオメタンだけでなく、セルロースナノファイバー、リグニンエポキシ樹脂やリグニンファイバー等の有用製品を製造した。

 


(a)


(b)


環境保全型前処理(高活性水蒸気+粉砕)システムを用いて製造された
(a)セルロースナノファイバーおよび(b)リグニンファイバー 

 

研究経緯等

【学術誌等への掲載状況】

1)Y. Nakamura, C. Asada, T. Sawada, Production of Antibacterial Violet Pigment by Psychrotrophic Bacterium RT102 Strain, Biotechnol. Biopro. Eng., Vol.8, No.1, pp.37-40, 2003.2.

2)F. Kobayashi, H. Take, C. Asada, Y. Nakamura, Methane Production from Steam-Exploded Bamboo, J. Biosci. Bioeng., Vol.97, No.6, pp.426-428, 2004.6.

3)C. Asada, Y. Nakamura, F. Kobayashi, Development of a Novel Pulping Method without Generating Wastewater Using a Sodium Hydroxide Pretreatment and Steam Explosion, J. Chem. Eng. Japan, Vol.38, No.2, pp.158-161, 2005.2.

4)C. Asada, Y. Nakamura, F. Kobayashi, Waste Reduction System for Production of Useful Materials from Un-utilized Bamboo Using Steam Explosion Followed by Various Conversion Methods, Biochem. Eng. J., Vol.23, No.2, pp.131-137, 2005.4.

5)C. Asada, Y. Nakamura, F. Kobayashi, Chemical Characteristics and Ethanol Fermentation of the Cellulose Component in Autohydrolyzed Bagasse, Biotechnol. Biopro. Eng., Vol.10, No.4, pp.346-352, 2005.8.

6)小林史尚, 浅田元子, 中村嘉利, 重金属汚染土壌のファイトレメディエーションと有価金属の回収, 化学工学論文集, Vol.31, No.6, pp.476-480, 2005.11.

7)C. Asada, Y. Kondo, C. Sasaki, Y. Nakamura, Bioconversion of Soy Sauce Residue Treated with Steam Explosion into Ethanol by Meicelase and Mucor indicus, J. Food Technol., Vol.8, No.4, pp.187-190, 2010.10.

8)C. Asada, A. Kita, C. Sasaki, Y. Nakamura, Ethanol Production from Disposable Aspen Chopsticks Using Delignification Pretreatments, Carbohydr. Polym., Vol.85, No.1, pp.196-200, 2011.4.

9)C. Asada, A. Asakawa, C. Sasaki, Y. Nakamura, Characterization of the Steam-Exploded Spent Shiitake Mushroom Medium and Its Efficient Conversion to Ethanol, Bioresour. Technol., Vol.102, No.21, pp.10052-10056, 2011.11.

10)C. Asada, C. Sasaki, Y. Uto, J. Sakafuji, Y. Nakamura, Effect of Steam Explosion Pretreatment with Ultra-High Temperature and Pressure on Effective Utilization of Softwood Biomass, Biochem. Eng. J., Vol.60, No.1, pp.25-29, 2012.1.

11)C. Sasaki, K. Sumimoto, C. Asada, Y. Nakamura, Direct Hydrolysis of Cellulose to Glucose Using Ultra-High Temperature and Pressure Steam Explosion, Carbohydr. Polym., Vol.89, No.1, pp.298-301, 2012. 6.

12)C. Sasaki, S. Hashimoto, C. Asada, Y. Nakamura, Evaluation of Buckwheat and Barley Tea Wastes as Ethanol Fermentation Substrates, J. Mat. Cycles Waste Manag., Vol.14, No.3, pp.206-211, 2012. 9.

13)C. Sasaki, R. Okumura, A. Asakawa, C. Asada, Y. Nakamura, Effects of Washing with Water on Enzymatic Saccharification and D-Lactic Acid Production from Steam Exploded Sugarcane Bagasse, J. Mat. Cycles Waste Manag., Vol.14, No.3, pp.234-240, 2012. 9.

14)C. Asada, R. Okumura, C. Sasaki, Y. Nakamura, Acceleration of Hericium erinaceum Mycelia Growth in Submerged Culture Using Yogurt Whey as an Alternative Nitrogen Source, Advan. Biosci. Biotechnol., Vol.3, No.7, pp.828-832, 2012.11.

15)C. Asada, K. Doi, C. Sasaki, Y. Nakamura, Efficient Extraction of Starch from Microalgae Using Ultrasonic Homogenizer and Its Conversion into Ethanol by Simultaneous Saccharification and Fermentation, Nat. Resour., Vol.3, No.4, pp.175-179, 2012.12.

16)Y. Noda, C. Asada, C. Sasaki, S. Hashimoto, Y. Nakamura, Extraction Method for Increasing Antioxidant Activity of Raw Garlic Using Steam Explosion, Biochem. Eng. J., Vol.73, No.1, pp.1-4, 2013.4.

17)C. Sasaki, M. Wanaka, H. Takagi, S. Tamura, C. Asada, Y. Nakamura, Evaluation of Epoxy Resins Synthesized from Steam-Exploded Bamboo Lignin, Ind. Crop. Prod., Vol.43, No.1, pp.757-761, 2013.5.

18)C. Sasaki, R. Okumura, C. Asada, Y. Nakamura, Steam Explosion Treatment for Ethanol Production from Pear Tree Prunings by Simultaneous Saccharification and Fermentation, Biosci. Biotechnol. Biochem., Vol.78, No.1, pp.160-166, 2014.1.

19)C. Sasaki, M. Ichitani, K. Kunimoto, C. Asada, Y. Nakamura, Extraction of Arbutin and Its Comparative Content in Branches, Leaves, Stems, and Fruits of Japanese Pear Pyrus pyrifolia cv. Kousui, Biosci. Biotechnol. Biochem., Vol.78, No.5, pp.874-877, 2014.5.

20)C. Sasaki, Y. Kushiki, C. Asada, Y. Nakamura, Acetone-Butanol-Ethanol Production by Separate Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) Methods Using Acorns and Wood Chips of Quercus acutissima as a Carbon Source, Ind. Crop. Prod., Vol.62, No.1, pp.286-292, 2014.12.

21)C. Asada, C. Sasaki, T. Takamatsu, Y. Nakamura, Conversion of Steam-Exploded Cedar into Ethanol using Simultaneous Saccharification, Fermentation and Detoxification Process, Bioresour. Technol., Vol.176, No.1, pp.203-209, 2015. 1.

22)C. Asada, S. Basnet, T. Otsuka, C. Sasaki, Y. Nakamura, Epoxy Resin Synthesis Using Low Molecular Weight Lignin Separated from Various Lignocellulosic Materials, Int. J. Biol. Macromol., Vo.74, No.1, pp.413-419, 2015.3.

23)C. Asada, C. Sasaki, T. Hirano, Y. Nakamura, Chemical Characteristics and Enzymatic Saccharification of Lignocellulosic Biomass Treated Using High-Temperature Saturated Steam: Comparison of Softwood and Hardwood, Bioresour. Technol., Vol.182, No.1, pp.245-250, 2015. 4.

24)A. Asakawa, M. Kohara, C. Sasaki, C. Asada, Y. Nakamura, Comparison of Choline Acetate Ionic Liquid Pretreatment with Various Pretreatments for Enhancing the Enzymatic Saccharification of Sugarcane Bagasse, Ind. Crop. Prod., Vol.71, No.1, pp.147-152, 2015.9.

25)S. Basnet, M. Otsuka, C. Sasaki, C. Asada, Y. Nakamura, Functionalization of the Active Ingredients of Japanese Green Tea (Camellia sinensis) for the Synthesis of Bio-Based Epoxy Resin, Ind. Crop. Prod., Vol.73, No.1, pp.63-72, 2015.10.

26)C. Sasaki, Y. Yoshida, C. Asada, Y. Nakamura, Total Utilization of Japanese Pear Tree Prunings: Extraction of Arbutin and Production of Bioethanol, J. Mat. Cycles Waste Manag., Vol.18, No.2, 385-392, 2016.4.

27)A. Asakawa, T. Oka, C. Sasaki, C. Asada, Y. Nakamura, Cholininum Ionic Liquid/Cosolvent Pretreatment for Enhancing Enzymatic Saccharification of Sugarcane Bagasse, Ind. Crop. Prod., Vol.86, No.1, pp.113-119, 2016.8.

28)A. Suzuki, C. Sasaki, C. Asada, Y. Nakamura, Characterization of Cellulose Nanofiber from Steam-exploded Japanese Cedar, BioResources, Vol.12, No.4, pp.7628-7641, 2017.8.

29)C. Sasaki, A. Kiyokawa, C. Asada, Y. Nakamura, Glucose and Valuable Chemicals Production from Cotton Waste Using Hydrothermal Method, Waste Biomass Valori., in press (https://link.springer.com/article/10.1007/s12649-017-0084-x).

30)C. Asada, C. Sasaki, Y. Nakamura, High Concentration Ethanol Production from Mixed Softwood Sawdust Waste, Waste Biomass Valori., in press (https://link.springer.com/article/10.1007/s12649-017-0073-0).

31)C. Asada, C. Sasaki, A. Suzuki, Y. Nakamura, Total Biorefinery Process of Lignocellulosic Waste Using Steam Explosion Followed by Water and Acetone Extractions, Waste Biomass Valori., in press (https://link.springer.com/article/10.1007/s12649-017-0157-x).

 

今後の展望(研究者からのコメント)

バイオマス(生物資源全般)より環境低負荷な総合プロセスでバイオ燃料と高付加価値化成品(高性能エコリファイナリーパルプや熱硬化性樹脂等)が製造されることによって石油依存型社会より脱却できる。